Journal: International Journal of Nanomedicine
Article Title: Inhibited Metastasis and Amplified Chemotherapeutic Effects by Epigene-Transfection Based on a Tumor-Targeting Nanoparticle
doi: 10.2147/IJN.S247567
Figure Lengend Snippet: Characterization of the nanoparticles. Notes: ( A ) SEM and TEM images of IPPD NPs and IPPD-PHF2 NPs (scale bars: TEM=100nm, SEM=200nm). The red arrows represented the plasmids adsorbed onto the surface of IPPD NPs, while the blue arrows represented the IPPD NPs. ( B ) Size distribution of IPPD-PHF2 NPs and zeta potential of PD, IPD, IPPD and IPPD-PHF2 NPs. ( C ) Safety concentrations of IPP NPs. ( D ) Standard curve of IR780 concentration, regression equation: Y=8.205X+0.0677, R square=0.9927, embedding rate of IR780≈16.5%. ( E ) Adsorption between IPPD NPs and PHF2, green: Doxorubicin, red: PHF2 dyed by Gel-Red. ( F ) Covalent binding between branched PEI and PLGA measured by FTIR, peak A: the typical double peaks range from 3400cm −1 to 3520cm −1 , peak B: the significant peak of primary amine in amido bond ranges from 1600cm −1 to 1640cm −1 .
Article Snippet: PHF2 primary antibody was purchased from Cell Signaling Technology (USA).
Techniques: Zeta Potential Analyzer, Concentration Assay, Adsorption, Binding Assay